【摘要】 目的:建立美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖的含量测定方法。方法:采用苯酚-硫酸法测定粘多糖的含量,采用正交试验设计考察ML-D中粘多糖的水解工艺和显色条件,采用单因素试验考察加热显色时间、苯酚和硫酸的用量。结果:ML-D中粘多糖的最佳水解工艺为:盐酸浓度6 mol/L,固液比为1∶1,水解时间1 h;最佳显色条件:苯酚2 mL,硫酸20 mL,加热显色时间10 min。结论:该法操作简便,精密度高,重复性好,可作为美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖含量的测定方法。更多还原

【Abstract】 Objective: To establish a method for determining of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from Periplaneta americana.Methods: Phenol-vitriol colorimetry was used for determining content of glycosaminoglycan;orthogonal design L(934)was adopted to investigate hydrolysis process of glycosaminoglycan in ML-D;single factor experiment was used to investigate time of color,dosage of phenol and vitriolic.Results: For the best hydrolysis process of glycosaminoglycan in ML-D,the density of hydrochloric acid is 6 mol/L,Solid-liquid ratio is 1: 1,and time of hydrolysis is 1 h.There are the best conditions of color,the dosage of phenol and vitriolic respectively is 2 mL and 20 mL,heating time is 10 min.Conclusion: The method is simple and accurate with reproducibility;it can be used for the determining content of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from Periplaneta americana.更多还原