【摘要】 目的探讨骨髓间充质干细胞(MSCs)对大鼠佐剂性关节炎(AA)的治疗、干预作用。方法收集培养扩增SD大鼠骨髓MSCs。SD大鼠注射弗氏完全佐剂造模之后行MSCs治疗及干预。造模后定期测量大鼠体质量、踝关节周长和后足爪体积。治疗后第21天,行四肢关节X线片及病理检查。结果模型大鼠经MSCs治疗后关节肿胀减轻,X线片示骨质破坏明显修复。病理HE染色见关节滑膜病变减轻。干预组经MSCs治疗,原发性炎症和继发病变均不明显,关节X线片和病理检查均未见明显异常,与模型组大鼠相比,体质量、足爪体积以及踝关节周长差异有统计学意义(P<0.05)。结论 MSCs治疗可有效减轻并逆转弗氏完全佐剂诱导的大鼠AA的进展。更多还原

【Abstract】 Objective To explore the mechanisms of action about how marrow mesenchymal stem cells(MSCs)treat and intervene the adjuvant arthritis(AA)of rats.Methods MSCs of SD rats which had been cultured and cloned would be collected.Freund′s complete adjuvant was administered in rats.The rats from the model group were given MSCs treatment and intervention.During the treatment period,those rats′weights,ankle circumferences and hind paw volumes were measured.On the 21st day of the treatment,limb-joints X-ray examination and pathological examination were taken.Results After using MSCs to treat adjuvant arthritis of rats,the joint swelling of the MSCs group were relieved,and the limb-joints X-ray showed the destructive bones had apparent restoration.Pathological microscopic examination of HE staining ankle joint showed joint synovial lesions significantly reduced.In the MSCs intervention group,rats′primary inflammation and secondary lesions were not obvious,meanwhile,joint X-ray and pathological examination showed no obvious abnormalities.Compared with the model group,the differences of rats′weight,paw volume and ankle circumference had statistically significant(P<0.05).Conclusion MSCs could relieve and reverse the progression of AA.更多还原