【摘要】 目的研究中国献血者中感染HBV的基因型及亚型的分布特征,分析献血者中HBV基因型、亚型与献血者HBV血清学特征及病毒学特征的关系。方法从2008~2010年5家合作血液中心(血站)HBsAg确证阳性献血者中随机选取245份样本进行研究;通过S区扩增测序结合多对型特异性引物巢式PCR法分析HBV基因型,通过PreS/S区扩增测序或全基因组扩增测序分析HBV基因亚型。用SPSS 11.0统计软件分析各地HBV基因型、基因亚型的分布,比较献血者中HBV基因型和亚型分布与慢性乙型肝炎患者的区别,分析HBV基因型、基因亚型与人口特征,病毒学特征和血清学特征的关系。结果 245份样本中成功确定基因型228例,成功确定基因亚型200例。5地献血者感染中HBV的主要基因型为B型和C型,B型流行率高于C型,未见E-H型;基因亚型依次为B2,C2,D1,A1;在昆明献血者中发现了B基因型的1种新亚型B9;C型和C2亚型中的HBeAg阳性率分别显著高于B型和B2亚型中的HBeAg阳性率。结论我国献血者中感染的HBV的主要基因型为B型和C型,B型高于C型,且具有流行率增高的趋势,C型献血者中HBV复制活性高于B型。C型中的主要亚型为C2,B型中的主要亚型为B2。更多还原

【Abstract】 Objective To study the distribution of genotype and subgenotype of HBV in Chinese blood donors and analyze the relationship between the genotype and subgenotype of HBV and the serological and viral characteristics of HBV. Methods A total of 245 blood samples were selected randomly from HBsAg positive blood donors of 5 geographicallydiverse blood centers in China. The S region of HBV was amplified by semi-nested PCR and HBV genotype was determined. The PreS plus S region or the whole genome of HBV was amplified and sequenced and HBV subgenotype was determined. The SPSS 11. 0 software was used to compare the distri-bution of HBV genotype and subgenotype in Chinese blood donors and in patients and to analyze the relationship between the genotype and subgenotype of HBV and the serological and viral characteristics of HBV in blood donors. Results Of the 245 samples,228( 93. 8%) were genotyped successfully and 200( 81. 6%) were subgenotyped successfully. The major genotypes of HBV in Chinese blood donors were B and C with the former more prevalent than the latter. E-H genotypes of HBV were not found in this study. The major subgenotypes were B2,C2,D1 and A1 in sequence. A new subgenotype B9 was detected in Kunming. The prevalence of HBeAg in C genotype and C2 subgenotype is higher than that in B genotype and B2 subgenotype. Conclusion HBV genotype B was the most frequent strain circulating in HBV infected Chinese blood donors,followed by genotype C. There is a trend that genotype B is becoming more prevalent in Chinese blood donors.更多还原