【摘要】 旨在探索过度训练对大鼠红细胞的损伤及牛磺酸的保护作用机制。以过度训练大鼠和喂食丙二醛(MDA)大鼠为模型,研究二者对红细胞形态、红细胞膜脂质的微粘度及红细胞膜骨架蛋白的影响,并分别喂食Tau来拮抗过度训练大鼠和喂食MDA大鼠。结果表明,过度训练大鼠和喂食MDA大鼠都有相似但不同程度地改变红细胞形态,并使红细胞膜流动性显著降低和红细胞溶血度显著升高,在膜蛋白电泳上,都产生了新的高分子量电泳条带(HMWA)。以牛磺酸(Tau)喂食各对照组后,能有效地逆转由于过度训练和喂食MDA对红细胞形态和结构的损伤。以上实验结果提示:MDA可能是导致过度训练后红细胞结构损伤的重要因子,可能是引起运动性疲劳的重要因素,Tau抗疲劳作用的机制可能是拮抗MDA对机体细胞的损伤。更多还原

【Abstract】 Objective: This paper aims to probe the damaging mechanisms of erythrocytes in the processing of overtrained condition in rats and the possible protection ways of erythrocytes by taurine. Methods: Using the animal models of over-trained rats and MDA fed rats,this paper tested the red blood cell physiological indexes including the shape of erythrocyte,the Micro-viscosity of erythrocyte membrane lipid and the structure of erythrocyte membrane proteins. In this paper two groups were fed with taurine to be investigated protection effects. Results: The shapes of erythrocyte were changed in both the over-trained rats and the MDA fed rats. Besides,the cell membrane lipid micro-viscosity decreased significantly,and the hemolysis degrees of red blood cells increased significantly. A new electrophoresis band( high molecular weight aggregation,HMWA) was imaged in SDS-PAGE. After taurine was added in each control group,the conditions of erythrocyte shapes,the fluidity of membrane lipid and the hemolysis degrees were reversed,and the HMWA was inhibited. Conclusion: MDA maybe act as one of the important factors leading to the exercise-induced fatigue and over-trained. The protective mechanism of taurine may employed from MDA impairing the cell membrane structures.更多还原