【摘要】 目的探讨宫颈鳞状细胞癌中磷酸化细胞外信号调节激酶(P-ERK)1/2和基质金属蛋白酶(MMP)-9的表达与临床病理关系。方法采用免疫组织化学方法检测P-ERK1/2和MMP-9在30例慢性宫颈炎、45例宫颈上皮内瘤变(CIN)和58例宫颈鳞癌患者组织中的定性表达情况。结果 P-ERK1/2和MMP-9在慢性宫颈炎、CIN及宫颈鳞癌组织中的阳性率分别为0和0、28.9%和24.4%及77.6%和65.5%,3组间差异均有统计学意义(χ2=54.393,P=0.003;χ2=40.968,P=0.005)。宫颈鳞癌患者中,P-ERK1/2和MMP-9在临床Ⅱ～Ⅲ期组、G3组、有淋巴结转移组和肿瘤直径>4 cm组的阳性率分别明显高于临床Ⅰ期组(P=0.015,P=0.002)、G1～G2组(P=0.013,P=0.017)、无淋巴结转移组(P=0.017,P=0.021)和肿瘤直径≤4cm组(P=0.008,P=0.004)。P-ERK1/2与MMP-9在宫颈鳞癌组织中的阳性表达呈正相关(χ2=8.955,P=0.006)。结论 P-ERK1/2和MMP-9的阳性率随宫颈病理改变的程度加深而增加。P-ERK1/2和MMP-9在宫颈鳞癌中过表达可能促进了宫颈鳞癌的浸润及其淋巴结转移,二者在宫颈鳞癌浸润转移过程中可能有协同作用。更多还原

【Abstract】 Objective To study the expressions and clinical significances of p-extracellular regulated kinase(P-ERK)1/2 and matrix metalloproteinase-9(MMP-9)in cervical squamous cell carcinoma.Methods The expressions of P-ERK1/2 and MMP-9 in 30 cases with chronic cervicitis,45 cases with cervical intraepithelial neoplasia(CIN),and 58 cases with cervical squamous cell carcinoma were detected by immunohistochemical method.Results The positive rates of P-ERK1/2 and MMP-9 in chronic cervicitis,CIN,and cervical squamous cell carcinoma were 0 and 0,28.9% and 24.4%,77.6% and 65.5%,respectively,showing significant differences among these three groups(χ2 = 54.393,P=0.003;χ2=40.968,P=0.005).The positive rates of P-ERK1/2 and MMP-9 in patients at clinical stages Ⅱ-Ⅲ,at G3,with lymphatic metastasis,or with a tumor diameter greater than 4 cm were significantly higher than those at clinical stage Ⅰ(P=0.015,P=0.002),at G1-G2(P=0.013,P=0.017),without lymphatic metastasis(P=0.017,P=0.021),or with a tumor diameter less or equal than 4 cm in cervical squamous cell carcinoma(P=0.008,P=0.004).There was a positive correlation between P-ERK1/2 and MMP-9 in cervical squamous cell carcinoma(χ2=8.955,P=0.006).Conclusions The expressions of P-ERK1/2 and MMP-9 increase gradually with the progression of cervical squamous cell carcinoma.The overexpressions of P-ERK1/2 and MMP-9 may promote the infiltration of cervical squamous cell carcinoma and lymphatic metastasis,druing which these two enzymes may exert their effets in a synergistic manner.更多还原