【摘要】 目的:建立金钮扣药材中有效成分绿原酸HPLC含量测定方法,为其药材质量评价和资源合理利用提供科学依据。方法:采用高效液相色谱法对不同产地、不同采收期和不同部位金钮扣中绿原酸含量进行比较分析。色谱柱为Agela Promosil C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液(10∶90),流速为1.0mL/min,检测波长为327nm,柱温为35℃。结果:绿原酸的浓度在0.1000～200.0μg/mL范围内与峰面积呈良好的线性关系r,=0.9999,平均回收率为99.8%,RSD为0.71%。不同产地、不同采收期和不同部位金钮扣中绿原酸含量存在一定差异。结论:所建立的方法准确可靠、灵敏度高、专属性强,可有效控制金钮扣药材的质量,为金钮扣资源合理利用提供科学依据。更多还原

【Abstract】 Objective: To develop a HPLC method for determination of chlorogenic acid in Solanum torvum and provide a scientific basis for evaluating the quality and reasonable utilization of the herb.Methods: HPLC was used to quantitatively determine the chlorogenic acid content in Solanum torvum from different origin,different harvest time and different part.The assay was performed on a Agela Promosil C18(4.6 mm×250 mm,5μm)column and eluted with a mobile phase consisted of acetonitrile-0.1% phosphoric acid solution at a flow rate of 1.0 mL/min.The column temperature was set at 35 ℃.The detection wavelength was 327 nm.Results: The calibration curve was linear within the range of 0.1000～200.0 μg/mL(r=0.9999).The average recoveries were 99.8%(RSD=0.71%).The contents of chlorogenic acid in Solanum torvum were different from different origin,different harvest time and different part.Conclusion: The method is reliable,accurate and specific.It can be used for quality control of Solanum torvum and reasonable utilization of the herb.更多还原