【摘要】 目的:对2005年-2010年霍乱弧菌监测中分离的25株疑似菌株进行系统鉴定。方法:分离培养、血清学试验、系统生化鉴定(VITEK32)、PCR检测其分型及毒素基因、药敏试验。结果:25株菌鉴定为霍乱弧菌的有21株,与霍乱弧菌发生交叉凝集4株;病例监测主要为O139霍乱弧菌、外环境监测主要为O1群霍乱弧菌;病例监测菌株携带霍乱弧菌毒素基因(ctxAB)、其他菌株均不携带霍乱弧菌毒素基因(ctxAB)。药敏实验结果对诺氟沙星和环丙沙星敏感,对其他抗生素有不同程度的耐药。结论:武汉市霍乱弧菌在病例监测中主要为O139群霍乱弧菌产毒株、外环境监测中主要为O1群霍乱弧菌非产毒株,不同型别菌株存在不同程度耐药性。更多还原

【Abstract】 Objective:25 suspected strains of Vibrio cholerae isolated between 2005 and 2010 in the Vibrio cholerae surveillance program were identified and analyzed by different kinds of assays.Methods:The isolates were analyzed by conventional diagnostic methods and PCR assays for their molecular typing and virulence-related genes,and drug sensitivity test.Results:In 25 suspected strains of Vibrio cholerae,there were 21 vibrio cholerae isolates and four strains cross-agglutinating with Vibrio cholerae,respectively.Vibrio Cholerae Serogroup O139 came mostly from patients,and Vibrio cholerae Serogroup O1 was mostly isolated from natural circumstances.Six strains of Vibrio cholerae Serogroup O139 were positive for ctxAB gene,and others were negative.The drug sensitivity tests indicated that all suspected strains of Vibrio cholerae were sensitive to norfloxacin and ciprofloxacin,and exhibited varying degrees of resistance to other antibiotics.Conclusion:The suspected strains of Vibrio cholerae isolated from patients were mostly Vibrio Cholerae Serogroup O139,and Vibrio cholerae Serogroup O1 came mostly from natural circumstances.All suspected isolates showed varying degrees of resistance to antibiotics.更多还原