【摘要】 目的:建立不同产地野菊花中绿原酸、3,4-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸的含量测定。方法:采用HPLC方法,色谱柱为Diamonsil(4.6 mm×250 mm,5μm),甲醇-0.1%磷酸溶液做流动相进行梯度洗脱,检测波长326 nm,柱温25℃,流速0.8 mL.min-1。结果:绿原酸、3,4-二咖啡酰奎尼酸和3,5-二咖啡酰奎尼酸的线性范围分别为1.00～20.00,4.64～92.80,2.233～44.66 mg.L-1;加样回收率分别为98.97%,99.14%;99.39%,RSD分别为1.81%,1.66%,1.36%(n=6)。结论:方法操作简便,分析速度快,结果准确,重复性好,可用于野菊花中绿原酸、3,4-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸的含量测定。更多还原

【Abstract】 Objective:To determine the content of chlorogenic acid,3,4-O-dicaffeoylquinic acid,3,5-O-dicaffeoylquinic acid in Chrysanthemi Indici Flos of different areas.Method: HPLC was used.The chromatographic separation was performed on a Diamonsil C18(4.6 mm × 250 mm,5 μm) column eluted with gradient mobile phase of methanol-0.1%H3PO4,the detection wavelength was set at 326 nm and column temperature was set at 25 ℃.Result: Nice linear relation between the area and content when the amount was within 1.00-20.00,4.64-92.80,2.233-44.66 mg·L-1.The recoveries were 98.97%,99.14% and 99.39% respectively,RSD was 1.81%,1.66% and 1.36%(n=6) respectively.Conclusion: The method is accurate,reproducible and suitable to determine these active components in Chrysanthemi Indici Flos.It can be used for quality control of Chrysanthemi Indici Flos.更多还原