【摘要】 目的初步评价登革病毒IgM抗体免疫层析试剂条的检测效果。方法应用4种血清型登革病毒重组外膜抗原,组装登革病毒IgM抗体通用型和分型免疫层析试纸条,检测登革病毒IgM抗体,结果与捕获法ELISA比较。同时观察分型试纸条的血清学分型效果。结果以4型抗原混合组装的通用型试纸条检测结果与捕获法ELISA检测结果比较,二者的总体符合率为90.56%,检测结果无显著差异(P>0.05)。在与其它传染病交叉反应测试中,通用型检测试剂较捕获法ELISA具有更低的交叉反应。分型结果表明,除登革I型外,其它型别试纸条均能检出非对应型别的IgM抗体。结论利用登革病毒重组抗原组装的通用型IgM抗体免疫层析试纸条与捕获法ELISA检测效果相近,具有实际应用的潜力,但需要进一步做大样本的评估。而分型试纸条分型效果不佳,需要进一步的改进。更多还原

【Abstract】 This study conduct the evaluation on performance of dengue IgM antibody immuno-chromatography strip(ICS).Two forms of ICS,the general ICS assembled with combined recombinant antigens(rAg) of four serotypes and the typing ICS assembled with distinct serotype of rAg,were applied to detect the IgM antibody and type the infection of virus.The result of the general ICS test coincided well with that of IgM antibody capture ELISA(Mac ELISA) test with a 90.56% overall identity,and the results of this two methods showed no significant difference(P>0.05).The ICS showed lower cross-reactivity than Mac ELISA when detecting the IgM antibody against other infection agents.The typing strips showed intertype cross-reactivity excepting for the D1 strip.This study implies the potential application of the general ICS in laboratorial diagnosis of dengue virus infection,but its efficacy needs further evaluation with large scale of specimens.And the typing ICS needs further improvement.更多还原