【摘要】 为了准确测定饲料中7种β2-兴奋剂,建立了超高效液相色谱-串联质谱检测方法。样品经酸性有机混合溶剂提取、固相萃取净化浓缩后,在多反应监测模式下,用带电喷雾离子源(ESI)的串联质谱仪对样品进行定性和定量分析。该方法下各化合物定量检出限为50μg/kg。在50、100、200μg/kg的添加浓度下,回收率在71%～103%之间,相对标准偏差在5.7%～9.8%之间。本方法快速、准确、灵敏度高,适合饲料中福莫特罗、苯乙醇胺A、克仑特罗、莱克多巴胺、沙丁胺醇、特布它林、西马特罗的同时快速检测。更多还原

【Abstract】 A method of ultra performance liquid chromatograghy-electrospray tandem mass spectrometry(UPLC-MS/MS) was studied for determination of β2-agonist in feed.The sample was extracted by 0.1 mol/L hydrochloric acid-methanol mixed solvent,purified and enriched with cationic solid phase extraction column,and which was separated by liquid chromatography on C18 column with mobile phase of 0.1% formic acid in methanol(gradient elution),then analyzed in UPLC-MS/MS with electrospray ion source(ESI) under multi-reaction monitor mode(MRM).The quantification was performed with external standard method.The method has a lower limit of quantitation 50 μg/kg.Spike recoveries of three concentration addition levels(50,100,200 μg/kg) ranged in 71%—103% with relative standard deviations in 5.7%—9.8%.The method is fast,accurate and sensitive.It is suitable for rapid qualitative and quantitative detection of β2-agonist in animal feed.更多还原