【摘要】 目的探讨结直肠癌远处转移患者免疫基因表达的变化趋势。方法从16例结直肠癌患者的原发癌肿瘤组织中提取mRNA,采用基因芯片技术检测8例有肝脏转移和无肝脏转移的结直肠癌患者的免疫基因表达。结果与无肝脏转移的结直肠癌患者相比,有肝脏转移的结直肠癌患者的肿瘤组织中有11条免疫基因即羧基肽酶D、高亲和力IgE Fc受体γ链、低亲和力IgG FcⅢa受体、游离脂肪酸受体2、白细胞介素-2γ链、受体型蛋白酪氨酸磷酸酶C、补体B因子、人类白细胞抗原复合物(HLA)-DMA、HLA-DMB、HLA-DQA1和颗粒酶B均表达下调。涉及功能变化包括免疫细胞的生长激活、信号传递、肿瘤免疫原性、细胞因子、受体、补体、肿瘤细胞凋亡等方面。结论在结直肠癌肝转移患者中免疫基因的表达普遍下调,从多种途径影响机体免疫功能的发挥,使癌细胞逃避机体免疫系统杀灭而在远处目标脏器生长、繁殖。更多还原

【Abstract】 Objective To investigate the change of immunologic gene expression in cases of colorectal cancer with liver metastasis.Methods The total RNAs were extracted from tumor tissues of original lesions in 16 patients with colorectal cancer,DNA microarray was used to examine the change of immunologic gene expression in colorectal cancer patients with or without liver metastasis.Results Compared with samples without liver metastases,the expressions of 11 immunologic genes obviously down-regulated in the tumor tissues of colorectal cancer patients with liver metastasis,including:carboxypeptidase D;Fc fragment of IgE,high affinity Ⅰ receptor for gamma polypeptide;Fc fragment of IgG,low affinity Ⅲa receptor(CD16a);free fatty acid receptor 2;interleukin 2 receptor gamma;protein tyrosine phosphatase receptor type C;complement factor B;major histocompatibility complex,class Ⅱ,DM alpha;major histocompatibility complex,class Ⅱ,DM beta;major histocompatibility complex,class Ⅱ,DQ alpha 1;granzyme B.The functions involved the growth and activation of immunologic cell,signal transduction,cell apoptotic,cell factors,receptors,complement,apoptotic,and immunogenicity of tumor cell.Conclusions Down-regulation of a various of immunologic gene expression in colorectal cancer patients with liver metastasis inhibits the function of immunology,and tumor cells escaped the destruction of immunology system results in metastasis.更多还原