【摘要】 应用田口设计对保加利亚乳杆菌静息细胞转化合成共轭亚油酸进行优化。在单因素试验基础上,应用Minitab设计2水平4因素正交试验,采用田口设计方法分析正交试验结果。结果表明,静息细胞浓度、pH值、温度、亚油酸浓度对共轭亚油酸产量影响均不显著。软件预测产共轭亚油酸的最佳工艺为静息细胞浓度7%、pH值为5.7、温度32℃、亚油酸浓度1.7g/L,此时共轭亚油酸产量为142.3mg/L,与软件预测值140.63mg/L相吻合,表明优化方案达到了预期效果,且具有良好的稳定性。更多还原

【Abstract】 The production of conjugated linoleic acids by resting cells of Lactobacillus bulgaricus was optimized using Taguchi design.On the basis of single factor test,the L8(24) of orthogonal experiment was designed using Minitab software,and the results were analyzed using Taguchi design.The results indicated that the effects of the concentration of resting cells,pH value,temperature and the concentration of linoleic acids on the production of conjugated linoleic acids were insignificant.Minitab software predicted the optimal production of conjugated linoleic acids were as follows: the concentration of resting cells 7%,pH value 5.7,temperature 32℃ and the concentration of linoleic acids 1.7g/L.Under these conditions,the yield of conjugated linoleic acids reached 142.3mg/L,which corresponded to the predicted value(140.63mg/L).This indicated that optimum program is good.更多还原