【摘要】 目的:检测信号转导与转录激活因子5(STAT5)、血管内皮生长因子(VEGF)和表皮生长因子受体(EGFR)在非小细胞肺癌(NSCLC)中的表达情况,探讨三者与NSCLC临床病理特征之间的关系及三者彼此的关系。方法:采用免疫组化(SP法)检测STAT5、VEGF和EGFR在68例NSCLC及26例癌旁正常对照组织中的表达情况。结果:(1)STAT5、VEGF及EGFR在NSCLC组织中的阳性表达明显高于肺正常组织(P<0.05);(2)STAT5的阳性表达与NSCLC组织学类型有关,在腺癌中的表达明显高于鳞癌,与组织分化程度、TNM分期及淋巴结转移无关;(3)VEGF的阳性表达与NSCLC的分化程度、淋巴结转移和TNM分期有关(P<0.01),与NSCLC的组织学类型无关(P>0.05);(4)EGFR的阳性表达与NSCLC的TNM分期和淋巴结转移有关(P<0.01),与NSCLC的组织学类型和分化程度无关(P>0.05);(5)Spearman相关分析显示STAT5、VEGF和EGFR在NSCLC中的阳性表达两两比较(秩和检验方法)均呈正相关。结论:STAT5、VEGF、EGFR可能在肺癌的发生、侵袭和转移中起重要的作用,抑制STAT5信号通路可望成为非小细胞肺癌的治疗靶点。更多还原

【Abstract】 Objective:To examine the expression of STAT5,VEGF and EGFR in NSCLC and to study their relationship.Methods:STAT5,VEGF and EGFR proteins were detected in formalin-fixed paraffin-embedded tissue from 68 non-small cell lung cancer tissues and 26 normal lung tissues by SP immunohistochemical method.The levels of their expression and their relationship with STAT5,VEGF and EGFR were analyzed.Results:(1)The positive rates of STAT5,EGFR and VEGF in NSCLC were dramatically higher than that in the normal lung tissues(P<0.01);(2)The expression of STAT5 were significantly correlated with histological types of NSCLC(P<0.01),and were higher in adenocarcinomas than in squamous carcinomas;(3)The expression of VEGF were significantly correlated with histological grading,TNM stage and lymph node metastasis(P<0.05),but were not correlated with the histological types of NSCLC(P>0.05).(4)The expression of EGFR were significantly correlated with TNM stage and lymph node metastasis(P<0.05),but were not correlated with the histological types and grading of NSCLC(P>0.05).(5)There was significantly positive association among STAT5,VEGF and EGFR.Conclusion:The high expression of STAT5,VEGF and EGFR in NSCLC play san important role in occurrence,invasion and metastasis of NSCLC.The inhibition of STAT5 signal access may become a targets for cancer therapy.更多还原