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一株拮抗纹枯病菌放线菌的筛选及鉴定

Screening and identification of antagonistic actinomycete against Rhizoctonia solani

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【作者】 崔宇辉王媛媛汪鹏荣高爱同曹丽凌蒋冬花

【Author】 CUI Yuhui,WANG Yuanyuan,WANG Pengrong,GAO Aitong,CAO Lilin,JIANG Donghua(College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China)

【机构】 浙江师范大学化学与生命科学学院

【摘要】 从采自金华市各地的68份土样中分离得到了放线菌406株,以水稻纹枯病菌为指示菌,利用平皿对峙法筛选到42株具有较好拮抗作用的放线菌菌株.其中,Sh-43菌株的拮抗作用最强,抑菌带宽度为28.3mm.根据培养特征、形态特征、生理生化特征及16S rDNA序列比对分析,鉴定Sh-43菌株为吸水链霉菌(Strep-tomyces hygroscopicus).

【Abstract】 Four hundred and six actinomycetes were isolated from sixty eight soil samples which were collected from different areas of Jinhua.Forty-two strains had been proved to have antagonistic activity against Rhizoctonia solani by confronting cultivation on PDA plates.Among them,the strain Sh-43 exhibited the strongest antibiosis capacity with an inhibition zone of 28.3 mm.The strain Sh-43 was identified as Streptomyces hygroscopicus on the cultural,morphological,physiological,biochemical characteristics and 16S rDNA gene sequence analysis.

【基金】 浙江省公益性应用研究计划项目(2010C32076)
  • 【文献出处】 浙江师范大学学报(自然科学版) ,Journal of Zhejiang Normal University(Natural Sciences) , 编辑部邮箱 ,2012年04期
  • 【分类号】S476.1
  • 【被引频次】5
  • 【下载频次】102
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