【摘要】 目的:建立2种藤黄酸异构体的高效液相含量测定方法。方法:采用色谱柱SunFire(Waters)C8(2.1 mm×150mm,3.5μm),流动相为乙腈-甲醇-0.3%三氟乙酸溶液(36∶37∶27),检测波长360 nm,流速0.3 mL·min-1,柱温28℃。结果:R-藤黄酸,S-藤黄酸线性关系方程分别为Y=2.87×106X-2.24×105,r=0.999 9;Y=3.31×106X-1.44×105,r=0.999 9。平均回收率分别为100.0%,100.9%;RSD分别为2.1%,2.5%(n=6)。藤黄药材供试品中R-藤黄酸和S-藤黄酸的平均质量分数为30.06%,21.45%。结论:本方法简便、稳定,可用于藤黄中藤黄酸2种异构体的鉴定与含量测定。更多还原

【Abstract】 Objective: To establish a method for determing the content of two isomers containd in Garcinia hanburyi by HPLC.Method: Chromatographic column of SunFire(Waters)C8(2.1 mm×150 mm,3.5 μm)was adopted,with acetonitrile-methanol-0.3% trifluoroacetic acid(36∶37∶27)as the mobile phase.The detection wavelength was 360 nm,the flow rate was 0.3 mL·min-1,and the column temperature was 28℃.Result: The linear regression equation of r-gambogic acid was Y=2.87×106X-2.24×105,r=0.999 9.The linear regression equation of S-gambogic acid was Y=3.31×106X-1.44×105,r=0.999 9.The average recoveries were 100.0% and 100.9%,with RSD being 2.1% and 2.5%(n=6),respectivley.The average contents of two gambogic acid in G.hanburyi were 30.06% and 21.45%,respectively.Conclusion: The method was so convenient and stable that it can be used for identification and content determination of two isomers containd in G.hanburyi.更多还原