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正交试验优选杜仲多糖提取工艺

Optimization of the Extraction Process for Eucommia ulmoides Polysaccharides by Orthogonal Test

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【作者】 史秀玲高银辉梁海英周程艳

【Author】 SHI Xiu-ling,GAO Yin-hui(Leting County Hospital of Hebei Province,Hebei Leting 063600,China) LIANG Hai-ying(Tangshan Fengrun District Hospital of TCM,Hebei Tongshan 064000,China) ZHOU Cheng-yan(Shenyang Pharmaceutical University,Shenyang 110016,China)

【机构】 河北乐亭县医院唐山市丰润区中医院沈阳药科大学

【摘要】 目的:优选杜仲多糖的提取工艺。方法:用普通水提醇沉法和微波提取法分别提取杜仲多糖,以提取温度、提取时间、料液比、提取次数为考察因素,以多糖提取率为评价指标,采用正交试验优选工艺。结果:普通水提醇沉法的最佳工艺为温度120℃、时间2 h、料液比1∶8、次数3次;微波提取法的最佳工艺为温度50℃、时间45 min、料液比1∶20、次数3次。结论:微波提取法对杜仲多糖的提取有辅助作用,较普通水提醇沉法更优。

【Abstract】 OBJECTIVE:To optimize the extraction process for Eucommia ulmoides polysaccharides(EOP).METHODS:Aqueous extraction and microwave extraction were used for the extraction of E.ulmoides polysaccharides.The extraction technology of E.ulmoides polysaccharides was optimized by orthogonal test with extraction temperature,extraction time,solid-liquid rate and extraction times as factors using extraction rate as index.RESULTS:The optimum process conditions with aqueous extraction were as follows:extraction temperature of 120 ℃,extraction time of 2 h,solid-liquid ratio of 1:8,extracting for 3 times.The optimum process conditions with microwave extraction were as follows:extraction temperature of 50 ℃,extraction time of 45 min,solid-liquid ratio of 1:20,extracting for 3 times.CONCLUSION:Microwave-assisted extraction is helpful to extract E.ulmoides polysaccharides,and it is better than aqueous extraction.

【基金】 唐山市科学技术研究与发展指导计划项目(111302098a)
  • 【文献出处】 中国药房 ,China Pharmacy , 编辑部邮箱 ,2012年03期
  • 【分类号】TQ461
  • 【被引频次】13
  • 【下载频次】849
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