【摘要】 目的:探讨游泳+跑台的混合运动训练对D-半乳糖所致阿尔茨海默病(AD)模型大鼠海马内β位淀粉样蛋白42和裂解酶1(Aβ42、BACE1)的影响。方法:将16只雄性SD大鼠随机分为安静注射组(C组)和运动注射组(T组),所有大鼠连续8周腹腔注射D-gal造阿尔茨海默病模型大鼠,同时,T组大鼠连续进行8周的游泳和跑台(均每周3次,交替进行)相结合的混合运动训练。运用WesternBlot法检测大鼠海马Aβ42蛋白表达量,运用RT-PCR法检测大鼠海马BACE1mRNA和IGF-1mRNA的表达量。结果:①与C组相比,T组大鼠海马内Aβ42蛋白表达量显著减少,具有极显著性差异(P<0.01);②C组比T组大鼠海马内BACE1mRNA表达量多,具有显著性差异(P<0.05);③与C组相比,T组大鼠海马内胰岛素源生长因子(IGF)-1mRNA表达增加,具有极显著性差异(P<0.01)。结论:游泳和跑步混合运动训练能延缓AD的发展进程,这可能与运动训练上调了AD大鼠海马内IGF-1mRNA表达有关。更多还原

【Abstract】 Objective:To study the effects of swimming and treadmill training on hippocampal β-amyloid precursor protein 42(Aβ42)and it’s cleaving enzyme1(BACE1)in rat model of D-galactose induced Alzheimer’s disease(AD).Method:Sixteen SD male rats were randomly divided into sedentary injection group(C)and exercise injection group(T).All of the rats were received injection of D-galactose into abdominal cavity continuously for 8 weeks to make AD animal models.Group T were in addition given mixed training(swimming and treadmill training alternatively each 3 times per week)for 8 weeks.The levels of hippocampal Aβ42 protein of rats were inspected by Western blot method.The expression levels of hippocampal BACE1 mRNA and insulin derived growth factory(IGF)-1mRNA of rats were inspected by RT-PCR technique.Result:①Compared with group C,expression of Aβ42 protein in group T reduced significantly in hippocampus of rats(P<0.01).②Expression of BACE1 mRNA was higher in group C than that in group T(P<0.05).③Compared with group C,up-regulation of expression of hippocampal IGF-1mRNA in group T was founded(P<0.01).Conclusion:This investigation demonstrates that swimming and treadmill training mixed exercise is a simple behavioral intervention,can inhibit the progression of AD.It may be related with the up-regulation of expression of hippocampal IGF-1mRNA by exercise training.更多还原