【摘要】 目的声动力治疗(sonodynamic therapy,SDT)是通过超声波激活肿瘤细胞内聚集的声敏剂治疗肿瘤的一种新方法。本实验用Chlorin e6为声敏剂,观测其在S180肉瘤荷瘤小鼠肿瘤组织的富集情况及联合超声对S180肉瘤的杀伤作用。方法 S180肉瘤荷瘤小鼠腹腔注射Chlorin e6后,共聚焦显微镜观测其在肿瘤的富集情况,18 h后超声处理(1.0 MHz,180 s),每2天测量肿瘤大小,第15天剥离肿瘤进行质量比较。结果腹腔给药后18 h,Chlorin e6在肿瘤组织和肌肉组织含量达峰值,且二者含量差异最明显,此时为最佳的超声辐射时间。单独使用超声照射(0.4～1.6 W/cm~2)或单独注射Chlorin e6(10～40mg/kg)对小鼠S180肉瘤无明显杀伤作用。Chlorine6联合超声可明显抑制肿瘤生长,抑瘤作用随超声强度(0.4～1.6 W/cm~2)和Chlorin e6质量分数(10～40 mg/kg)的增加而增强。结论 Chlorin e6在S180肉瘤荷瘤小鼠具肿瘤靶向聚集性,联合超声介导的SDT对小鼠S180肉瘤有明显杀伤作用。更多还原

【Abstract】 Objective The sonodynamic therapy(SDT) is a promising new approach for cancer therapy by activating a tumor-localizing sonosensitizer with ultrasound.The purpose of this study was to explore whether Chlorin e6, a sonosensitizer,can accumulate selectively in the S180 sarcoma of mice and to assess its antitumor effects of SDT. Methods After intraperitoneal injection of Chlorin e6,the concentration of Chlorin e6 in tumor and muscle was measured by laser scan confocal microscope(LSCM) at different time points.18 h later,the 1.0 MHz ultrasound irradiation ’was treated for 180 s.The tumor size was recorded every 2 days and the tumor was weighted on the 15th day after treatment.Results After intraperitoneal injection of Chlorin e6,the concentrations of Chlorin e6 in tumor and muscle tissue reached to a peak at 18 h.The difference of Chlorin e6 concentration between tumor and muscle tissue was greatest at 18 h,which suggested that it was the optimal timing for SDT.Both ultrasound(0.4～1.6 W/cm~2)or Chlorin e6 (10～40 mg/kg)alone had no significant antitumor effects.However,the combination of ultrasound with Chlorin e6 (SDT) exerted significant antitumor effects.This antitumor effects was intensity-dependent for ultrasound and dosedependent for Chlorin e6.Conclusions Chlorin e6 can accumulate selectively in the S180 sarcoma of mice and exerted antitumor effects as a sonosensitizer activated by ultrasound.更多还原