【摘要】 目的研究中国不同居群野生玉竹种质资源的遗传多样性。方法选取我国11个不同居群的野生玉竹样品和1个居群的栽培样品进行ISSR分析,Popgene 32及Ntsyspc-2.1软件分析处理数据。结果通过筛选得到10条ISSR引物,扩增得到115条带,其中108条为多态性条带,多态性条带百分率为93.38%;Nei’s基因多样性(H)为0.432 1±0.084 1,Shannon信息指数(I)为0.619 7±0.100 5,遗传距离(GD)变异为0.128 9～0.496 5;利用UPGMA法构建分子树状图,可将12个居群聚合为两大类。结论不同产地野生玉竹种间存在较高的多态性,遗传多样性较为丰富;野生玉竹的遗传多样性高于栽培居群的遗传多样性,可能与栽培方式和环境因素有关;药用植物玉竹对环境变化的适应能力强,地理来源相同的野生居群和栽培居群先行聚合,与其种质间的亲缘关系较近有关;聚类结果可以部分反映地理分布的特点,为种质资源的鉴定打下良好基础。更多还原

【Abstract】 Objective To investigate the genetic diversity of wild Po1ygonatum odoratum from diferent habitats of China.Methods Eleven wild P.odoratum samples from different habitats and one cultivar were selected for ISSR analysis.Popgene 32 and Ntsyspc-2.1 softwares were used for data analyses.Results Ten ISSR primers were screened and 115 bands were amplified,among which 108 bands were polymorphic(PPB = 93.38%).Nei’s gene diversity(H) was 0.432 1 ± 0.084 1,Shannon’s genetic diversity(I) was 0.619 7 ± 0.100 5,and genetic distance(GD) among 12 populations ranged from 0.128 9 to 0.496 5.UPGMA was used for establishing dendrogram tree and 12 populations were clustered into two groups.Conclusion Higher polymorphism is observed from P.odoratum among different habitats.The genetic diversity of wild P.odoratum is higher than that of wild population,which is related with culture methods and environmental factors.P.odoratum has high adaptability to the environmental changes.Results show that wild and cultural P.odoratum from the same habitat cluster in advance,which is related to the near phylogenetic relationship among species.Cluster analysis could partly reflect the characteristic of geographical distribution and provide the reference for the identification of germplasm.更多还原