【摘要】 目的:观察己烯雌酚诱导大鼠垂体瘤成瘤的稳定性,为后继研究提供实验基础。方法:60只标准Wistar-Furth大鼠随机分成己烯雌酚注射组、灭菌花生油注射组和对照组,每组20只,观察处理后大鼠一般情况、体重及行为学改变,并分别于4周、8周、和12周处死大鼠,观察成瘤情况和垂体重量变化,HE染色及免疫组化染色的方法鉴别肿瘤类型及CD31表达。结果:处理后2周,己烯雌酚注射组大鼠体重增长明显较其余2组缓慢,并出现脱毛、行动缓慢等表现。在相应时点处死大鼠,实验组出现肿瘤形成,4周、8周和12周成瘤率分别为30%、90%和100%,其余2组成瘤率为0。HE染色可见异常细胞。免疫组化鉴别均为催乳素(PRL)腺瘤,部分混杂促肾上腺皮质激素(ACTH)。实验组垂体组织无论成瘤与否,CD31阳性表达率与对照组相比差异显著。成瘤与否跟大鼠性别无关。结论:腹腔注射己烯雌酚诱导Wistar-Furth大鼠垂体瘤模型成瘤效果确切稳定。在成瘤过程中,部分指标出现动态变化,可以作为进一步研究的模型。更多还原

【Abstract】 AIM:To establish a stable pituitary tumor model by diethylstilbestrol(DES) induction in Wistar rats.METHODS: Wistar-Furth rats were randomly divided into DES treatment group,arachis oil treatment group and control group.The behavior changes and body weight of the rats were recorded.Ten rats in DES group and 5 in each of the other 2 groups were sacrificed after 4,8 and 12 weeks,respectively.The weight and the histological and morphological changes of the pituitary gland,serum prolactin(PRL) level,and expression of CD31 were detected.RESULTS: Trichomadesis and behavior changes such as slowdown and anorexia were observed in a majority of rats in DES group 2 weeks after treatment.The increase in body weight of the rats in DES group was significantly slower than that in the other 2 groups.Tumor formation was detected in DES group,and abnormal cells were found after HE staining in despite of the sexes.CD31 expression in DES group was significantly higher than that in the other 2 groups.Serum PRL and the weight of pituitary gland were higher in DES group.CONCLUSION: Pituitary tumor model induced by DES in Wistar-Furth rats is a stable and convenient pituitary tumor model.更多还原