【摘要】 目的:初步探讨血管内皮生长因子(VEGF)在卵巢组织自体移植中的应用价值。方法:将健康成熟未交配过的20只雌性SD大鼠随机分为2组,A组去势后自体异位移植新鲜卵巢组织,B组去势后自体异位移植经VEGF处理过的新鲜卵巢组织。移植后饲养4周,观察动情周期的恢复情况和卵巢组织的形态学变化,测定血清中的激素水平。结果:B组大鼠的雌二醇水平明显高于A组,其差异有统计学意义(P<0.05);B组移植卵巢组织的体积明显大于A组,其差异有统计学意义(P<0.05);对形态正常的各级卵泡统计分析,生长卵泡数目在各组之间的差异没有统计学意义。结论:在卵巢组织移植过程中使用VEGF,可以减少卵巢组织的缺血再灌注损伤,有助于促进卵巢组织功能的恢复。更多还原

【Abstract】 AIM: To evaluate the effect of vascular endothelial growth factor(VEGF) on the ovarian tissues during transplantation.METHODS: Twenty regularly cycling rats were randomly divided into 2 groups: the rats in group A received fresh autologous ovarian transplantation without VEGF administration(n=10);the rats in group B received fresh autologous ovarian transplantation with VEGF administration(n=10).Four weeks after transplantation,the ovarian tissues and sera of the rats were collected for histological examination and determination of the hormone levels.RESULTS: The serum concentration of estradiol in group B was significantly higher than that in group A(P<0.05).Four weeks after transplantation,we found that the ovarian tissues were significantly developed,and the average size of the ovaries in group B was significantly larger than that in group A(P<0.05).CONCLUSION: The ovarian tissues with VEGF administration develop better than those without VEGF administration.VEGF can increase the graft vascularity and the percentage of surviving tissues.更多还原