【摘要】 目的:探讨使用电化学发光免疫法(ECLIA)测定血清胰岛素及C-肽时标本溶血程度及溶血标本放置时间对测定结果的干扰。方法:将检测标本人为干预成5种不同溶血程度的溶血标本,置于室温20℃环境下,分为4个时间段(0min、30min、60min、120min)分别采用电化学发光免疫法测定溶血和非溶血标本的胰岛素及C-肽浓度并作分析。结果:标本溶血可使胰岛素浓度降低,胰岛素浓度测定与标本溶血程度及放置时间呈负相关;标本溶血对电化学发光免疫法测定C-肽无干扰。结论:测定胰岛素时要绝对避免溶血;在标本分析前质量控制不确定情况下C-肽释放水平曲线比胰岛素释放水平曲线在临床上更具可信性。更多还原

【Abstract】 Objective:To approach specimen hemolysis degree and hemolytic specimen waiting period interfering with the testing results while using electrochemiluminescence immunoassay(ECLIA) to test serum insulin and C-peptide.Methods:We artificially interfered with the test specimen into 5 hemolysis specimens with different hemolysis degrees.And we placed them in indoor environment(20℃).Using ECLIA to test and analyze insulin and C-peptide degree of hemolytic and non-hemolytic specimens separately during 4 different time quanta(0,30,60,120 min).Results:Specimen hemolysis can lower insulin concentration.Insulin concentration testing and specimen hemolytic degree and waiting period are in negative correlation.Specimen hemolysis has no influence on using Electrochemiluminescence Immunoassay to test C-peptide.Conclusion:Definitely avoid hemolysis during insulin test.Under the circumstance of unsure quality control before specimen analysis,C-peptide releasing horizontal curve is more reliable in clinical practices than insulin releasing horizontal curve.更多还原