【摘要】 应用高效液相色谱法同时测定丁苯乳膏中丁酸氢化可的松和盐酸苯海拉明含量。色谱条件为:色谱柱:XTerra RP C18(250 mm×4.6 mm,5μm);流动相:甲醇-乙腈-0.5%醋酸铵溶液(35∶27∶38);流速:1.0 mL.min-1;检测波长:232 nm。丁酸氢化可的松及盐酸苯海拉明在125～250μg.mL-1及25～50μg.mL-1范围内线性关系良好,表明应用HPLC法可同时测定该制剂丁酸氢化可的松及盐酸苯海拉明的含量。更多还原

【Abstract】 This study determined simultaneously the contents of hydrocortisone butyrate and diphenhydramine hydrochloride in Dingben Cream with an HPLC method,in which the chromatographic column was XTerra RP C18(250 mm×4.6 mm,5 μm),the mobile phase was composed of methanol-acetonitrile-0.5% solution of ammonium acetate(35∶27∶38),the flew rate was at 1.0 mL·min-1 and the detecting wavelength was at 232 nm.The linear ranges of hydrocortisone butyrate and diphenhydramine hydrochloride were 125~250 μg·mL-1 and 25~50 μg·mL-1,respectively.which indicated that the method can be used to determine simultaneously the contents of hydrocortisone butyrate and diphenhydramine hydrochloride in preparations.更多还原