【摘要】 目的分离制取罗汉果叶黄酮,并与Cu配位形成Cu配合物,分别测定两种样品的癌细胞抑制活性,验证金属配位的影响效果。方法通过高速逆流色谱分离法(HSCCC)分离罗汉果叶粗提物,从中分离得到山奈酚-3,7-O-α-L-二鼠李糖苷(黄酮苷KR)。同时以黄酮苷KR作为配体,合成Cu配合物,并对其结构进行了表征分析,探讨黄酮苷KR及其Cu配合物的抗肿瘤活性。结果在0.2 mmol/L的浓度下,黄酮苷KR对卵巢癌细胞SK-OV-3、乳腺癌细胞MCF-7、肝癌细胞株HepG-2和胃癌细胞SGC-7901的抑制率分别为13.3%、13.7%、11.0%和20.5%,而黄酮苷KR的Cu配合物的抑制率则为44.2%、27.9%、27.6%和44.2%。结论黄酮苷KR的Cu配合物的抗肿瘤活性比黄酮苷KR更好,说明黄酮苷KR与金属的结合有利于发挥其潜在的协同效应。更多还原

【Abstract】 Objective To isolate and prepare flavonoid from Siraitia grosvenorii(Swingle) C.Jeffrey leaves,to synthesize Cu(Ⅱ) complex and study the effects of metal complex by determing the anti cancer activities of flavonoid and its complex.Methods Kaempferol-3,7-O-α-L-dirhamnopyranoside(KR) was separated from S.grosvenorii leaves by using high-speed counter-current chromatography(HSCCC).At the same time,the Cu(Ⅱ) complex of KR were synthesized and characterized.Lastly,the anticancer activities of them were determined.Results The inhibition rates of flavonoid KR at the concentration of 0.2 mmol/L to ovarian cancer SK-OV-3,breast MCF-3,liver cancer HepG-2 and gastric cancer SGC-7901 cell lines were 13.3%,13.7%,11.0% and 20.5%,respectively.And the Cu(Ⅱ) complex of KR were 44.2%,27.9%,27.6% and 44.2%,respectively.Conclusion The Cu(Ⅱ) complex has higher anticancer activity.The Cu(Ⅱ) can exert the potential synergy effect with KR.更多还原