【摘要】 目的:观察Th22细胞在原发免疫性血小板减少症(ITP)患者外周血中的变化。方法:用流式细胞术分别检测ITP初诊组、治疗后完全反应(CR)组及正常对照组外周血中Th22细胞所占比例。用酶联免疫吸附实验(ELISA)分别检测各组外周血中IL-22、TGF-β、TNF-α、IL-6的表达水平。用半定量RT-PCR分别检测各组外周血中IL-22 mRNA的表达水平。并逐层分析比较。结果:ITP初诊组和治疗后组Th22细胞比例、外周血中IL-22、TNF-α、IL-6的表达水平以及IL-22mRNA的表达水平均明显高于正常对照组(P<0.01),治疗后组Th22细胞比例、IL-22、TNF-α、IL-6、IL-22 mRNA的表达水平均低于初诊组(P<0.05);ITP初诊组和治疗后组TGF-β水平分别为(3.27±1.02)ng/L、(5.41±1.69)ng/L明显低于正常对照组(9.65±2.78)ng/L(P<0.01),且初诊组TGF-β的水平低于治疗后组(P<0.05)。结论:ITP患者体内Th22细胞数量增加、TNF-α、IL-6的表达升高、TGF-β的表达减低。更多还原

【Abstract】 AIM: To investigate the change of Th22 cells in the peripheral blood of the patients with primary immune thrombocytopenia(ITP) and evaluate the significance of Th22 cells in ITP.METHODS: The proportion of Th22 cells in the peripheral blood of ITP patients before therapy(group 1),ITP patients in complete response after therapy(ITP-CR,group 2) and healthy donors(group 3) was evaluated by flow cytometry.The cytokines IL-22,TGF-β,TNF-α and IL-6 of each group were measured by ELISA.The level of IL-22 mRNA of each group was examined by RT-PCR.RESULTS: The proportion of Th22 cells and the levels of IL-22,TNF-α,IL-6 and IL-22 mRNA in group l and group 2 were significantly higher than those in group 3(P<0.01).The proportion of Th22 cells and the levels of IL-22,TNF-α,IL-6 and IL-22 mRNA in group 2 were lower than those in group 1(P<0.05).But the level of TGF-β in group l [(3.27±1.02) ng/L] and group 2 [(5.41±1.69) ng/L] was significantly lower than that in group 3 [(9.65±2.78) ng/L](P<0.01),and the level of TGF-β in group 1 was lower than that in group 2(P<0.05).CONCLUSION: In ITP patients,the number of Th22 cells and the levels of TNF-α and IL-6 increase,and the level of TGF-β decrease.更多还原