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Effects of β-TCP Ceramics on Osteoblast Cellular Proliferating,Mineralization and Osteocalcin Expression

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【作者】 齐志涛； 张启焕；

【Author】 QI Zhitao1,2, ZHANG Qihuan1*, ZHENG Qiang3, DAI Honglian2, WANG Zisheng1, QIU Ming1, LI Shipu2 (1. School of Chemical and Biological Engineering, Yancheng Institute of Technology, Yancheng 224051, China; 2. The Bio-material Research and Engineering Center, Wuhan University of Technology, Wuhan 430070, China; 3. Yancheng Tianbang Feed Technology Co., Ltd, Yancheng 224056, China )

【机构】 School of Chemical and Biological Engineering, Yancheng Institute of Technology； The Bio-material Research and Engineering Center, Wuhan University of Technology；

【摘要】 After co-cultrured osteoblast with β-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that β-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that β-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that β-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.

【Abstract】 After co-cultrured osteoblast with β-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that β-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that β-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that β-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.

【关键词】 β-TCP ceramics； osteoblast； cellular proliferating； mineralization； osteocalcin；
【Key words】 β-TCP ceramics； osteoblast； cellular proliferating； mineralization； osteocalcin；
【基金】 the Research Fund of Key Labortary for Advanced Technology in Environmental Protection of Jiangsu Province (No. AE201037);the Foundation for Talent Recruitment of Yancheng Institute of Technology (No. XKR2011007);the “973” Chinese National Key Fundamental Research and Development Program (No. G1999064701)
  • 【文献出处】 Journal of Wuhan University of Technology(Materials Science Edition) ,武汉理工大学学报(材料科学版)(英文版) , 编辑部邮箱 ,2012年01期
  • 【分类号】R687.3;R318.1
  • 【被引频次】1
  • 【下载频次】46
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