【摘要】 研究用高效液相色谱法建立荷叶指纹图谱的分析方法。采用依利特Sino Chrom色谱柱(4.6 mm×200mm,5μm),以乙腈-缓冲溶液(H3PO4-NaH2PO4,0.1 mol/L,pH 2.5)为流动相,梯度洗脱,流速1.5 mL/min,柱温30℃,检测波长254 nm。用上述方法确定了10批不同品种荷叶的12个共有峰,各色谱峰分离效果良好,精密度、稳定性、重复性试验中各共有峰相对保留时间和相对峰面积的RSD均小于3%,符合指纹图谱要求。该方法精密度、稳定性和重复性均较好,为荷叶的质量控制标准提供了参考。更多还原

【Abstract】 The analytical method of Lotus leaves’ fingerprint chromatography was set up by high performance liquid chromatography.The chromatographic conditions were as follows a YILITE Sino Chrom(4.6 mm×200 mm,5 μm)with CH3CN-Buffer solution(H3PO4-NaH2PO4,0.1 mol/L,pH 2.5) as mobile phase in a gradient mode.The flow rate was 1.5 mL /min,and the column temperature was 30 ℃.The detecting wavelength was set at 254 nm.12 common peaks were found in the fingerprint of 10 batches of Lotus leaves.This method is accurate and steady with a good repeatability and providing a reference a reference for quality control of Lotus leaves.更多还原