【摘要】 建立了婴幼儿配方奶粉中左旋肉碱含量的高效液相色谱-质谱联用测定方法。采用C4色谱柱(4.6mm×250 mm,5μm)进行分离,以甲醇-水(10∶90)为流动相,柱温25℃,流速1.0 mL.min-1,进样量为10μL,柱后分流比为1∶3,质谱采用多离子反应监测(MRM)方式进行检测,正离子模式,定量离子为m/z 162.2→103.1。在优化条件下,样品在10 min内完成分析,左旋肉碱在0.40～10.0 mg.L-1范围内线性关系良好(r=0.999 3)。方法的加标回收率为87%～106%,检出限(S/N=3)和定量下限(S/N=10)分别为39.0、116.9 ng。该方法操作简便、灵敏度高、重复性好,可用于婴幼儿配方奶粉中左旋肉碱的测定。更多还原

【Abstract】 A high-performance liquid chromatography-mass spectrometric method was developed for the determination of L-carnitine in infant formula milk powder.After pretreatment procedure,the sample was separated on a C4(4.6 mm×250 mm,5 μm) column using methanol-water(10 ∶ 90) as mobile phase with a flow rate of 1.0 mL·min-1,a column temperature of 25 ℃ and injection volume of 10 μL.The split ratio of post column was 1 ∶ 3.The target compound was detected under multiple reaction monitoring(MRM) mode with positive ion using m/z 162.2→103.1 as the quantitation ions.Each analysis was completed within 10 min.A good linear calibration for L-carnitine was obtained in the range of 0.40-10.0 mg·L-1 with a correlation coefficient of 0.999 3.The spiked recoveries were in the range of 87%-106%.The limit of detection(S/N=3) and limit of quantitation(S/N=10) of L-carnitine were 39.0 ng and 116.9 ng,respectively.The proposed method was simple,sensitive and reproducible,and was suitable for the determination of L-carnitine in infant formula food.更多还原