【摘要】 目的:探讨黄芪注射液对H2O2损伤L6大鼠成肌细胞的修复。方法:取1、0.5、0.1、0.05mmol/L H2O2损伤成肌细胞建立模型,再取2 000、1 000、500、250、125、62.5mg/mL黄芪注射液实施保护。应用MTT法、流式细胞仪及荧光抗体Bax和Bcl-2检测,使用SPSS 15.0统计软件处理数据。结果:经0.1mmol/L H2O2损伤成肌细胞存活率由94.4±5.7%降至32.6±3.8%,凋亡率由0增至32.45±2.9%。黄芪注射液处理实验组细胞存活率64.5±4.8～82.2±9.6%较模型组32.6±3.8%增加,凋亡率也从32.45±2.9%减至2.96±0.5～9.29±2.7%,荧光抗体检测Bcl-2细胞数57.7±4.3～74.2±6.9,Bax细胞数42.3±4.0～60.2±5.1。结论:黄芪注射液对大鼠成肌细胞H2O2损伤经由p38MAPK信号通路实施保护。更多还原

【Abstract】 Objective:Astragalus injection was approached for repair of the L6 rat myoblast cells against H2O2 injury.Method:The concentration of 1,0.5,0.1,0.05mmol / L H2O2 were selected on myoblast cell injury models.The dose of Astragalus injection 2 000,1 000,500,250,125,62.5 mg / mL were added to protect damage cultured cells.MTT method,flow cytometry and fluorescent antibody of bcl-2 and bax were detected.The application of SPSS 15.0 statistical software was used to analyse data.Result:When myoblast cells was damaged by 0.1mmol/L H2O2,the rate of cell survival was reduced from 94.4±5.7% to 32.6±3.8%,and the rate of apoptosis was increased from 0 to 32.45±2.9%.The survival rate of experiment group treated by Astragalus injection was raised 64.5±4.8-82.2±9.6% with injury model 32.6±3.87% and the rate of apoptosis was decreased from 32.45±2.9% to 2.96±0.5-9.29±2.7%.Fluorescent antibody was detected for Bcl-2 cell number of 57.7±4.3-74.2 ±6.9,Bax cell count 42.3±4.0-60.2±5.1.Conclusion:The rat myoblast cells against H2O2 injury were protected by Astragalus injection via p38MAPK signaling pathway.更多还原