【摘要】 目的:构建X连锁视网膜劈裂(XLRS)三种不同类型突变体(点突变、缺失突变、插入突变),以便进一步构建不同类型的突变细胞模型。方法:根据NCBI gene数据库中XLRS1基因的CDS设计三对不同类型的引物,使用重叠PCR法获得突变片段,然后克隆入载体pLJM1-EGFP。结果:经测序验证,成功构建了三种不同类型突变体。结论:使用重叠PCR法构建三种不同类型突变体,为进一步研究XLRS1不同类型突变的致病机制奠定了基础。更多还原

【Abstract】 Objective: To construct three different types of mutations of XLRS1 for further modeling different types of mutant cell lines.Methods: Three different pairs of mutation primers were designed according to the CDS sequence of XLRS1 from the gene database of NCBI.Then three mutant fragments were obtained by overlapping PCR,which were further cloned into the vector pLJM1-EGFP.Results: Three types of mutant plasmids were verified by sequencing.Conclusions: Construction of three different types of mutants by overlapping PCR provided the foundation for investigating the pathogenesis of different types of XLRS1 mutatnts.更多还原