【摘要】 较系统地研究了茶叶粗多糖的初步纯化工艺。脱蛋白工艺中,三氯乙酸易降解茶多糖,Sevag法效果较好,而且以处理3次为好。比较大孔吸附树脂DM130、S8、HPD500、HZ801、AB8、NKA2和聚酰胺对茶多糖的脱色效果,结果表明聚酰胺的脱色效果和多糖保留率相对都较高,而且聚酰胺动态脱色效果比静态好。进一步脱色工艺中,DEAE-纤维素柱脱色效果好,但得率低,而且柱材料不能再生,而用Sephadex G50柱不仅效果好,柱材料还可以再生。因此,茶多糖初步纯化工艺可首先用Sevag法脱蛋白,然后用聚酰胺柱脱色,再用Sephadex G50进一步脱色,这样多糖的纯度可达到89%。更多还原

【Abstract】 In this paper,the technology of preliminarey purification of tea polysaccharide had been researched.For removing proteins,the method of Sevag,repeating 3 times,was better than that of TCA,which degraded tea polysaccharides.The decolor effects of polyamide was better than that of macroporous adsorption resins,such as DM130,S8,HPD500,HZ801,AB8 and NKA2,moreover,polyamide column should be used.For further removing pigments,Sephadex G50 column was selected,because the decolor effects of witch was better than that of DEAE-cellulose column.Therefore,the purification technology was:Using Sevag method,repeating 3 times,to removing protein,next using polyamide column to removing pigments,and then using Sephadex G50 to further removing pigments.Thus,the degree of purity of polysaccharides reached 89 %.更多还原