【摘要】 以鄂西宣恩野生刺梨汁为研究对象,采用顶空固相微萃取技术萃取游离态挥发性成分和Amberlite XAD-2树脂吸附、β-葡萄糖苷酶酶解释放O-糖苷键合态挥发性成分,并结合气相色谱-质谱联用分析技术,研究其游离态和O-糖苷键合态挥发性成分组成。结果表明:宣恩野生刺梨汁中游离态和O-糖苷键合态挥发性成分在组成和含量上差异较大;采用顶空固相微萃取技术结合气相色谱-质谱联用技术,鉴定出宣恩刺梨汁中游离态挥发性成分3 8种,含量较高的有(+)-柠檬烯、叶醇、丁酸乙酯、正己醇、苯乙烯、异戊酸乙酯、2,4-二叔丁基苯酚、松油烯、月桂烯、罗勒烯、丁酸-3-己烯酯、芳樟醇、辛酸、辛酸乙酯和双戊烯等;采用β-葡萄糖苷酶酶解释放结合气相色谱-质谱联用分析技术,鉴定出宣恩刺梨汁中O-糖苷键合态挥发性成分有38种,含量较高的有4-羟基肉桂酸、2′-羟基-4′,5′-二甲基苯乙酮、辛酸、肉桂酸、2,4-二叔丁基苯酚、3,4-二甲氧基肉桂酸、苯甲醇、己酸、3,4,5-三甲氧基苯酚、叶醇和阿魏酸等,其中有苯甲醇、苯乙醇、橙花醇、肉桂酸、香草酸、3-羟基己酸乙酯、丁香酚等33种物质在游离态组分中并不存在。更多还原

【Abstract】 Wide Rosa roxburghii juice from Xuan En,E’Zhou was extracted by headspace solid-phase microextraction(HSSPME) to obtain free volatile components,and adsorbed with Amberlite XAD-2 column and hydrolyzed with β-glucosidase to release O-glycoside-bound volatile components.Both volatile compounds were analyzed by gas chromatography-mass spectroscopy(GC-MS).The results indicated that there was a significant difference observed in the composition and contents of free volatile components and O-glycoside-bound volatile components.Totally 38 free volatile compounds and 38 O-glycosidebound volatile compounds were identified.The major free volatile compounds were(+)-limonene,leaf alcohol,ethyl butyrate,hexanol,styrene,ethyl isovalerate,2,4-di-tert-butylphenol,γ-terpinene,β-myrcene,β-ocimene,3-hexenyl butyrate,linalool,octanoic acid,ethyl caprylate and(-)-dipentene.Meanwhile,the major O-glycoside-bound volatile compounds were 4hydroxycinnamic acid,4’5’-dimethyl-2’-hydroxyacetophenone,octanoic acid,cinnamic acid,2,4-di-tert-butylphenol,3,4dimethoxycinnamic acid,benzyl alcohol,hexanoic acid,3,4,5-trimethoxyphenol,leaf alcohol and 4-hydroxy-3-methoxycinnamic acid.In addition,33 compounds were found only in the O-glycoside-bound volatile components,which were benzyl alcohol,phenylethyl alcohol,neroli,cinnamic acid,vanillic acid,3-hydroxybutyric acid ethyl ester,eugenol,and so on.更多还原