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糖化酶水解大豆异黄酮

Study onHydrolysis of Soybean Isoflavones by Saccharifying Enzyme

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【作者】 孙洁心张永忠

【Author】 Sun Jie-xin 1,Zhang Yong-zhong2,3 1(Heilongjiang Nongken Vocational College,Harbin 150025,China) 2(Department of Applied Chemistry,Northeast Agricultural University,Harbin 150030,China) 3(Key Laboratory of Soybean Biology,Ministry of Education,Harbin 150030,China)

【机构】 黑龙江农垦职业学院东北农业大学应用化学系教育部大豆生物学重点实验室

【摘要】 研究了糖化酶水解大豆异黄酮的技术工艺,利用糖化酶水解大豆异黄酮粉得到大豆异黄酮苷元。通过单因素试验对水解过程中的不同影响因素进行考察,运用正交试验优化了糖化酶水解大豆异黄酮的反应条件,优化结果为:水解温度55℃,pH值4.8,时间7 h,水解率可达98.18%。

【Abstract】 The technique of hydrolysis of soybean isoflavones by saccharifying enzyme was studied in this paper.The soybean isoflavone aglycon was obtained by hydrolysis.The different factors influencing the process of enzyme hydrolysis were studied through single factor tests.Reaction conditions of hydrolysis by saccharifying enzyme were optimized through orthogonal test.The results showed that the optimal hydrolysis conditions were as follows: the temperature was 55℃,the pH value was 4.8 and the time was 7 hours.The hydrolysis ratio could reach 98.18%.

【基金】 国家高技术研究发展计划(863计划)(编号:2008AA10Z331)
  • 【文献出处】 食品与发酵工业 ,Food and Fermentation Industries , 编辑部邮箱 ,2012年12期
  • 【分类号】TQ920.6
  • 【被引频次】6
  • 【下载频次】169
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