【摘要】 目的探讨人外周血血清瘦素及瘦素受体(LEPR)基因Gln223Arg多态性与胃食管反流病(GERD)的相关性。方法根据GERD症状诊断问卷联合胃镜检查确诊GERD,选取具有完整临床资料的GERD患者62例,分为反流性食管炎组(RE组)、非糜烂性反流病组(NERD组),同时选取健康体检者63例为对照组。采用酶联免疫法(ELISA)定量检测血清瘦素;采用限制性片段长度多态性聚合酶链反应法检测血清瘦素受体基因Gln223Arg多态性。结果 RE组、NERD组瘦素水平均明显高于对照组(P<0.05);RE组炎症的严重程度与瘦素水平呈正相关。RE组、NERD组瘦素受体基因Gln223Arg的GA+AA基因型频率和A等位基因频率均明显高于对照组(P<0.05)。GERD患者中GA+AA基因型的瘦素水平明显高于GG基因型(P<0.05)。结论 GERD的发生与血清瘦素水平升高及瘦素受体基因Gln223Arg多态性相关。更多还原

【Abstract】 Objective To investigate the relations between serum leptin and leptin receptor(LEPR) Gln223Arg polymorphism with gastro-esophageal reflux disease(GERD).Methods 62 patients with GERD were selected according to reflux disease questionnaire and gastroscope inspection and divided into the reflux esophagitis(RE) group and non-erosive reflux disease(NERD) group.In addition,63 healthy subjects were selected as the control group.The serum leptin levels were determined by ELISA,and LEPR Gln223Arg polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphisms assay.Results The levels of leptin in RE group and NERD group were both significantly higher than in control group(P<0.05);the degree of inflammation in RE group was positively correlated with leptin level.GA+AA genotype and A allele frequency of LEPR gene in RE group and NERD group were significantly higher than those in control group(P<0.05).The leptin in patients with GA+AA genotype was significantly higher than that in patients with GG genotype(P<0.05).Conclusion The serum leptin and the LEPR Gln223Arg polymorphism are associated with GERD.更多还原