【摘要】 运用PCR技术,扩增了梭鱼和鲻鱼线粒体16S rRNA和COⅠ基因片段,并比较分析了其种间的序列差异。获得16S rRNA基因的540～560 bp碱基序列,出现26个碱基的插入与缺失位点;获得COⅠ基因的602～604 bp碱基序列,出现2个插入缺失位点;16S rRNA和COⅠ基因的序列中G平均含量最低,且(A+T)含量高于(G+C)含量,与其他鱼类中的16S rRNA和COⅠ基因片段研究结果相一致。在16S rRNA基因片段中,梭鱼出现1种单倍型,鲻鱼为2种单倍型;在COⅠ基因片段中,梭鱼样品中检测到3个单倍型,鲻鱼样品中检测到5个单倍型。以Takifugu poecilonotus为外群,构建的NJ系统发育树,基于16S rRNA和COⅠ基因序列获得的NJ系统树基本一致,鲻鱼和梭鱼种内个体分别聚为一支,显示16S rRNA和COⅠ基因适合于梭鱼和鲻鱼的物种鉴定。更多还原

【Abstract】 The 16S rRNA and cytochrome oxidase subunit I(COⅠ) gene fragments of Liza haematocheila and Mugil cephalus were amplified by PCR,and the sequence variation among interspecies were analyzed.A total of 26 insertion or deletion of sites were detected for 16S rRNA;and 2 insertion or deletion of sites were detected for(COⅠ) gene.The average content of G was the lowest,and the content of(A+T) was higher than that of(G+C),which was similar to the results reported in other fish species.For 16S rRNA,one haplotype was observed in L.haematocheila and 2 haplotypes were observed in M.cephalus.As for COⅠ gene fragments,3 haplotypes were detected in L.haematocheila and 5 haplotypes were detected in M.cephalus.Takifugu poecilonotus was employed as outgroup,and the Neighbour Joining(NJ) phylogenetic tree was constructed.The results of NJ tree based on 16S rRNA and COⅠ were accordant.Intraspecific individuals L.haematocheila and M.cephalus were in a clade respectively.The present result indicated that 16S rRNA and COⅠwere applicable to molecular identification.更多还原