【摘要】 目的对从腹泻患者粪便中分离出的温和气单胞菌进行鉴定,并做毒素原性及致病性研究和建立药敏谱。方法碱性蛋白胨水增菌,接种碱性琼脂和TCBS平板分离培养,使用弧菌科细菌生化编码系统(第二代GYZ-9V系列)和API-20E生化编码系列对分离菌株进行鉴定,电子显微镜观察形态。毒素原性研究用溶血试验检测溶血素,小白鼠腹腔注射测定致病性,药敏试验采用纸片扩散法。结果从587例腹泻患者粪便标本分离出9株温和气单胞菌,毒素原性检测具有溶血素,致病性测定,该菌株毒力较强。所做的18种抗生素药敏试验,对13种敏感,2中度敏感,3种耐药。结论云南战区部队驻地存在温和气单胞菌感染,毒力试验证实,该分离菌是具有较强毒力的菌株,能使小白鼠发病死亡。药敏试验为部队驻地治疗此菌感染时的临床用药提供重要参考。更多还原

【Abstract】 Objective To differentiate the Aeromonas sobria isolated from diarrheal patients and the toxigenicity and pathogenicity of Aeromonas sobria.Methods Alkaline peptone was used for enrichment and cultured on alkaline agar and TCBS plate,then differentiated with vibriobacterial biochemical identification coding tube system(second generation GYZ-9v) and API-20E biochemical coding identification tube system.Kirby bauer method was used for drug resistance test.Results Nine Aeromonas sobria strains were isolated from 587 diarrheal patients and hemolysin was also detected by toxigenicity test.The pathogenicity of Aeromonas sobria was strong.The snesitivity of Aeromonas sobria to 18 antibiotics were tested and Aeromonas sobria were resistant to three antibiotics,relative sensitive to 2 anbiotics and sensitive to other 13 ones.Conclusion There is Aeromonas sobria contamination in Yunan Military Garrison and some of them possesses relatively strong pathogenicity that could lead to the death of mice.Attention be paid to clinical treatment of Aeromonas sobria infection as they are resistant to several antibiositcs.更多还原