【摘要】 目的建立在健康携带者分离的沙门菌的血清型及PFGE分子型别图谱库,探讨其多态相关性关系。方法将预防性健康体检分离获得的21株沙门菌鉴定培养,进行血清分型;将确定后的菌株用限制性内切酶Xba I消化酶切其基因组DNA,在脉冲场凝胶电泳仪进行DNA电泳,所得的分子型别图谱用Bionumerics5.1软件建立图谱数据库,并进行聚类分析。结果21株沙门菌分属于O:4(B)、O:9(D1)和O:3,10(E1)3个菌群,8种血清型;以德尔卑沙门菌为主,共7株占所有分离株的占33.3%,其次为阿贡纳沙门菌19.1%(4/21),鼠伤寒沙门菌14.3%(3/21)。经比对分析,18株沙门菌分为15个PFGE型别,菌株间的相似值在53.9%～100%之间,同一血清型别的沙门菌可对应多个PFGE型别。结论沙门菌的PFGE型别呈现多样性,脉冲场凝胶电泳对从业人员体检沙门菌有较高的分型能力,可为沙门菌的日常监测和相关疫情的处理提供重要的技术支撑。更多还原

【Abstract】 Objective To document the polymorphism of molecular type and serotype of Salmonella isolated from person in preventive physical examination by creating database aided with PFGE.Methods Twenty-one Salmonella stains were identified and serotyped by diagnostic serum.Then the total genomic DNA of the isolates was digested with restriction enzyme Xba I,and run pulsed-field gel electrophoresis(PFGE).The DNA fingerprints of different stains were analyzed by Bionumerics5.1 software to perform clustering.Results The 21 Salmonella strains belonged to three subgroup of O:4(B),O:9(D1),O:3,10(E1) and 8 serotype;S.Derby,accounted for 33.3%(7/21);S.Agona were 19.1%(4/21) and S.Typhimurium 14.3%(3/21);The DNA fingerprints of 18 Salmonella strains belonged to 15 PFGE pattern combinations.Similarity value of these strains was in the range of 53.9%～100%.One serotype Salmonella showed several PFGE patterns combinations.Conclusion PFGE pattern combinations of this study show polymorphism;The PFGE technology is suitable for molecular typing of Salmonella in preventive physical examination.更多还原