【摘要】 目的:研究Runx3基因CpG岛甲基化对胃癌病变的影响;探讨甲基化特异性聚合酶链法(MSP)和焦磷酸测序法(PS)对Runx3基因甲基化检测的特异性。方法:选取150例胃癌患者胃切除标本及50例无癌变胃黏膜标本,以MSP法和PS法同时检测其Runx3启动子的甲基化程度。结果:与正常胃黏膜相比,胃癌组织Runx3的甲基化程度差异有统计学意义(MSP法:67.3%vs 40.0%;PS法:76.0%vs 30.0%)。MSP结果显示,Runx3甲基化只与肿瘤大小相关,P<0.05。而PS法分析显示进展期胃癌的Runx3甲基化率高于早期胃癌;且其甲基化状态与肿瘤的大小(P=0.001)、Lauren分级(P=0.043)、浸润的深度(P<0.001)、淋巴结转移(P=0.004)及肿瘤TNM临床分期(P=0.003)等临床病理特征之间存在相关性。结论:Runx3启动子甲基化与胃癌临床病理特征密切相关,对于胃癌的早期诊断和治疗有重要意义。更多还原

【Abstract】 OBJECTIVE: To investigate the effects of Runx3 gene CpG island methylation on human gastric canner,and explore the specificity of methylation specific polymerase chain reaction(MSP) and pyrosequencing(PS) in Runx3 gene CpG island methylation detection.METHODS:Totally 150 specimen from patients with gastric canner and 50 normal specimen in our experiment were selected,and MSP and PS tests were used to detect the methylation status of Runx3 gene promoter.RESULTS: Compared to normal tissue,there were dramatec differences in methylation status of Runx3 gene CpG island in human gastric canner(MSP: 67.3% vs 40.0%;PS:76.0% vs 30.0%).MSP test demonstrated that Runx3 gene methylation was only related to tumor size(P<0.05).PS test showed that the extent of Runx3 gene methylation was higher in advanced gastric canner than that in early gastric canner,and the extent of methylation status of Runx3 gene was related to tumor size(P=0.001),Lauren’s classification(P=0.043),depth of invasion(P<0.001),lymph node metastasis(P=0.004),and TNM staging(P=0.003).CONCLUSION: Methylation status of Runx3 gene promoter is closely related to clinicopathological characteristics of human gastric canner,which plays an importand role in early diagnosis and therapy.更多还原