【摘要】 目的:研究牙周膜细胞(PDLCs)和骨髓基质细胞(BMSCs)共培养是否更利于牙周组织再生。方法 :用组织块法培养PDLCs,用全血贴壁法培养BMSCs,检测它们的成骨、成脂诱导分化能力。用Transwell小室共培养这两种细胞,通过3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)和实时荧光定量PCR(Real-time PCR)检测共培养后这两种细胞的增殖能力及部分基因的表达变化。结果:PDLCs和BMSCs具有多向分化潜能。共培养能促进它们各自增殖,上调Ⅰ型胶原(COLI)、碱性磷酸酶(ALP)、骨涎蛋白(BSP)、骨钙素(OCN)、核心结合因子2(RUNX2)的表达。结论:PDLCs和BMSCs共同运用作为种子细胞可能更有利于牙周组织再生。更多还原

【Abstract】 Objective: This study was designed to investigate whether the interaction of periodontal ligament cells(PDLCs) and bone marrow stromal cells(BMSCs) is related to better periodontal tissue regeneration.Methods: PDLCs and BMSCs were co-cultured by Transwell chambers.The co-cultured PDLCs and BMSCs were examined by MTT applying 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Real-time PCR assays.Results: PDLCs and BMSCs manifested multilineage differentiation potential.When co-cultured,proliferation rates of PDLCs and BMSCs raised.Gene expressions of COLI,ALP,BSP,OCN,RUNX2 were upregulated.Conclusion: It may be better to use PDLCs and BMSCs together for periodontal tissue regeneration.更多还原