【摘要】 目的比较正常大鼠和高果糖大鼠骨骼肌组织基因表达差异,探讨胰岛素抵抗的发病分子机制。方法实验大鼠分为正常对照组和高果糖组;从大鼠骨骼肌组织抽提mRNA,经逆转录分别用Cy3、Cy5荧光标记,获得两组动物来源cDNA探针;cDNA探针与4096条大鼠cDNA基因表达谱芯片杂交,扫描,重复2次实验,通过计算机数据处理。结果筛选出胰岛素抵抗差异表达的基因共140条,基因上调有62条,基因下调有78条。结论从骨骼肌组织中筛选出大量的差异表达基因,这些基因可能参加了胰岛素的发生发展过程。更多还原

【Abstract】 Aim To study the expression of insulin resistance-associated gene and to elucidate the molecular mechanisms in skeletal muscle tissue between fructose-fed rats and normal rats with cDNA microarray. Methods The rats were divided into two groups,one was normal and the other was fructose-rich chow.The mRNA was isolated and purified from skeletal muscle tissue of two groups.The mRNA from two kinds of tissue was reverse transcribed to cDNA with Cy3-dUTP and Cy5-dUTP separately to prepare hybridization probes.The mixed probes were hybridized to cDNA microarray.The microarray was scanned,analyzed and repeated for two times. Results Among the total 4096 tested genes,140 genes were differently expressed,62 up-regulated,78 down-regulated. Conclusions Gene expression profile is different from skeletal muscle tissue between fructose-fed rats and normal rats.The differential expression of genes may be related to the pathogenesis of insulin resistance.更多还原