【摘要】 目的:在大鼠无心跳供体(non-heart-beating donor,NHBD)肺移植动物模型上观察缺血后处理对NHBD供肺的保护作用。方法:将40只大鼠随机分成缺血后处理组(IPO组)和对照组(C组),每组10对,用"两套管一支架法"完成无心跳供体左肺原位移植。C组肺移植后直接开放肺动脉恢复灌注,而IPO组肺移植后给予5次1 min再灌/1 min再阻断的后处理措施,然后全面恢复再灌注。结果:IPO组与C组比较,NHBD肺移植术后肺水含量低、肺损伤程度轻,而肺顺应性、肺结构和功能保存更好。此外,IPO组移植术后供肺细胞凋亡程度和丙二醛(malondialdehyde,MDA)含量较C组低,而SOD含量较C组高。结论:缺血后处理可以减轻NHBD肺移植术后供肺缺血再灌注损伤,保存供肺结构和功能,还可抑制NHBD肺移植术后脂质过氧化反应和细胞凋亡。更多还原

【Abstract】 Objective:To explore the protective effects of ischemic postconditioning on non-heart-beating donor(NHBD) in rat NHBD lung transplantation model.Methods:Forty Sprague-Dawley rats were randomized into the ischemic postconditioning group(IPO group) and the control group(C group),10 pairs in each group in which left lung orthotopic transplantations from NHBDs were done with " two-cuff-one-stent technique".In the C group,perfusion was resumed by declamping pulmonary artery immediately after transplantation,whereas in the IPO group,5 cycles of 1-min reperfusion and 1-min reocclusion of pulmonary artery were applied as postcontioning before full recovery of perfusion.Results:Compared with the C group,water content of donor lungs was lower and pathological changes were milder in the IPO group,meanwhile compliance,structure and function of donor lungs were better preserved.Furthermore,the expression of cell apoptosis and MDA content in donor lungs were lower in the IPO group,while SOD content was higher.Conclusion:Ischemic postconditioning can reduce ischemic reperfusion injury of NHBD lung transplantation and preserve the structure and function of donor lungs.It can inhibit lipid peroxidation and cell apoptosis in NHBD lungs after transplantation.更多还原