【摘要】 研究了喹烯酮及其3种主要代谢物在猪肌肉、肝脏、肾脏、脂肪中的残留消除规律.猪按体质量20 mg/kg多剂量灌服给药,按既定时间点采集组织样品.组织样品用乙酸乙酯提取后经固相萃取小柱净化,液相色谱-串联质谱法测定样品中喹烯酮及其代谢物含量.结果表明:给药后猪几种组织中以肌肉中喹烯酮及其代谢物消除相对缓慢,药物含量以猪肝脏中3-甲基-苯乙烯酮-喹恶啉含量较高.3-甲基-苯乙烯酮-喹恶啉-N4-一氧化物在肌肉中的消除半衰期可达39.50 h.3-甲基-2-羧酸-喹恶啉仅能在肾脏中检测到.更多还原

【Abstract】 Residue depletion of quinocetone and three of its major metabolites in muscle,liver,kidney and fat of swine were determined in this study.Quinocetone was administered to 40 healthy cross-bread swine orally at dosage of 20 mg·kg-1 body mass twice a day for 5 consecutive days.Animals were slaughtered in groups and samples were taken at different time intervals after administration.The tissues were extracted with ethyl acetate,and cleaned up with HLB SPE column.A sensitive high-performance liquid chromatography-tandem mass spectrometry(LC-MS/MS) method was developed to determine quinocetone and its metabolites.Quinocentone and its metabolites were eliminated more slowly in swine muscle than those in other tissues.The highest drug levels occurred in liver for 3-methyl-styryl ketone-quinoxaline.The residue lasted the longest half-life of elimination for 39.50 h with 3-methyl-styryl ketone-quinoxaline-N4-monoxide in pig muscle.The 3-methyl-2-carboxylic acid-quinoxaline was detected only in kidney at very low concentration.更多还原