【摘要】 采用Alcalase碱性蛋白酶水解花生粕,设计单因素试验和正交试验考察水解时间、花生粕质量分数、酶用量、温度、pH等因素对水解度的影响,优化提取工艺。结果表明,最佳的水解条件为酶解温度60℃、pH 8.5,反应体系中花生粕和酶的质量分数分别为5%和7%,酶解时间4 h。在此条件下花生粕蛋白的水解度可达29.18%。更多还原

【Abstract】 Peanut meal was hydrolyzed with alcalase alkaline protease.Effects of reaction time,mass ratio of peanut meal,dose of enzyme,reaction temperature and pH on hydrolysis degree of peanut meal protein were studied by single factor tests and orthogonal experiment.The results showed that the optimum hydrolysis conditions were as follows,reaction temperature,60 ℃;pH 8.5;mass ratio of peanut meal,5%;enzyme dose,7%;and reaction time,4 h.Under these conditions,the hydrolysis degree of peanut meal protein was 29.18%.更多还原