【摘要】 重点介绍了利用定点突变技术对脂肪酶及酯酶性质的改造,包括酶的稳定性,活性,最适温度,最适作用pH以及对映选择性。综述了理性设计在脂肪酶及酯酶改造中的应用现状、存在问题以及前景。更多还原

【Abstract】 This paper focused on the engineering of lipase and esterase using site-directed mutagenesis,which can improve the stability,catalytic activity,optimal temperature,optimal pH and enantioselectivity of enzyme.The application status,problems and prospects of rational design in the engineering of lipase and esterase were reviewed.更多还原