【摘要】 目的探讨刀豆蛋白A(ConA)诱导小鼠急性肝损伤,形成肝纤维化的最佳成模时间与剂量。方法将BALb/c小鼠60只随机分为6组,即正常对照组和模型A、B、C、D、E组,每组10只。除正常对照组外,模型组分别经尾静脉注射8、10、12、14、16mg/kgConA。每周1次检测各组小鼠血清丙氨酸氨基转移酶(ALT)、总胆汁酸酶(TBA)、透明质酸酶(HA)水平,持续检测8周。8周后检测肝组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)水平。结果经不同剂量ConA诱导后随注射次数增多和持续时间延长,小鼠血清ALT、TBA、HA和肝组织匀浆中MDA、NO、SOD水平与正常对照组比较,差异有统计学意义(P<0.05)。结论小鼠经尾静脉注射剂量为12mg/kgConA第5周可获取理想的肝纤维化动物模型。更多还原

【Abstract】 Objective To explore optimal treat time and dosage of concanavalin A(ConA) to induce hepatic fibrosis in mouse model of acute liver damage.Methods 60 BALb/c mice were randomly divided into 6 groups,including normal control group and model group A,B,C,D and E,10 mice for each group.Mice of model groups were injected through caudal vein with ConA for 8,10,12,14 and 16 mg/kg of.Serum levels of alanine aminotransferase(ALT),total bile acid enzyme(TBA) and hyaluronidase(HA) were continuously detected in mice of every group for 8 weeks at frequency of once a week.Levels of malonaldehyde(MDA),superoxide dismutase(SOD) and nitrogen monoxidum(NO) in hepatic tissue homogenate were detected after 8 weeks.Results After induced by different dosages of ConA,with the increasing of injection frequency and prolonging of duration,serum levels of ALT,TBA and HA,and MDA,NO and SOD levels in hepatic tissue homogenate in mice of model groups were significantly different with those in normal control group(P<0.05).Conclusion Continuous infection of ConA with dosage of 12 mg/kg through caudal vein for 5 weeks could construct ideal mouse model with hepatic fibrosis.更多还原