【摘要】 用分光光度法研究缬氨酸与7,7,8,8-四氰基对苯醌二甲叉（TCNQ）的荷移反应。确定以pH=9.2的三酸缓冲溶液为介质,在50℃的水浴中加热30min,形成稳定的络合物,其λ_max=417nm,表观摩尔吸光系数ε=7.8×10³L·mol^-1·cm^-1,在1—9μg/mL的范围内符合比耳定律,回收率在96.7%—102.7%,相对标准偏差小于2.9%。更多还原

【Abstract】 The charge-transfer reaction of valine with 7,7,8,8-tetracyanoquinodimethane （TCNQ） was investigated by spectrophotometry.The experimental conditions were optimized and a new method for determination of valine has been developed.The stable charge-transfer complex was formed in Britton-Robinson buffer solution（pH=9.2） by heating in 50’C water bath for 30min.The apparent molar absorptivity of the complexεwas determined for 7.8×10³L · mol^-1 · cm^-1 at 417nm.Beer’s Law was obeyed in the range of 1—9μg/mL of valine.The recovery was 96.7%—102.7%,and the relative standard deviation was less than 2.9%.更多还原