【摘要】 目的观察妊娠期母鼠给予葡萄球菌肠毒B(SEB)对成年子代CD3+TCR Vβ8+T细胞的影响。方法在妊娠16 d时给予SD大鼠尾静脉注射15μg SEB,同时设立PBS对照组。孕鼠自然分娩后子代鼠生长至成年,流式细胞仪检测成年子代鼠胸腺及外周血中CD3+TCR Vβ8+T细胞;并观察成年子代鼠再次给予SEB时胸腺及外周血中CD3+TCR Vβ8+T细胞的应答变化。结果妊娠期母鼠给予SEB可导致雌、雄性成年子代鼠胸腺CD3+TCR Vβ8+T细胞的比例(雌性:1.760,雄性:1.098),较对照组的(雌性:2.714,雄性:2.088)明显减少(P<0.05),其外周血中CD3+TCR Vβ8+T细胞的变化与胸腺中类似。PBS组的雌雄性成年子代鼠给予SEB后其胸腺及外周血中CD3+TCR Vβ8+T细胞较同窝对照组明显减少(P<0.05);而SEB组的雌雄性成年子代鼠给予SEB后与其同窝PBS对照组比较,胸腺中CD3+TCR Vβ8+T细胞无变化(P>0.05),但外周血中CD3+TCR Vβ8+T细胞则明显增加(P<0.05)。结论妊娠期母鼠给予SEB改变其成年子代大鼠CD3+TCR Vβ8+T细胞对再次SEB刺激的应答方式。更多还原

【Abstract】 Objective To investigate the influence of maternal staphylococcal enterotoxin B(SEB) administration during pregnancy on CD3+ TCR Vβ8+T cells of adult offspring rats.Methods Pregnant maternal rats at gestational day(GD) 16 were injected intravenously with 15 μg SEB in 0.2 ml PBS(SEB group),and the control rats receive the same volume of PBS.Flow cytometry was used to determine the levels of CD3+ TCR Vβ8+T cells in both the thymus and peripheral blood of adult offspring rats and the response of these cells to a secondary SEB administration.Results Maternal SEB administration during pregnancy significantly decreased the percentages of CD3+ TCR Vβ8+T cells in the thymus in adult female(1.760-2.714) and male(1.098-2.088) offspring rats(P<0.05).The change of CD3+ TCR Vβ8+T cells in the peripheral blood was similar to that in the thymus.In the control adult offspring rats,SEB administration at adulthood significantly reduced the percentages of CD3+ TCR Vβ8+T cells in both the thymus and peripheral blood(P<0.05).But in SEB group,a secondary SEB administration in adult offspring rats significantly increased the percentage of CD3+ TCR Vβ8+T cells in the peripheral blood(P<0.05) but not in the thymus(P>0.05).Conclusion Maternal SEB administration during pregnancy can change the response of CD3+ TCR Vβ8+T cells of adult offspring rats to a secondary SEB administration.更多还原