乌司他丁对急性肺损伤大鼠肺组织HO-1表达的影响

【摘要】 目的乌司他丁对内毒素诱导大鼠急性肺损伤(acute lung jury,ALI)时血红素氧合酶(hemeoxygenase,HO)-1表达,从而为探讨乌司他丁在ALI中的应用提供理论依据。方法将48只健康雄性SD大鼠随机分为阴性对照组、ALI模型组、乌司他丁干预组和乌司他丁+氯血红素(Hm)组,每组各12只。模型组用5mg/kg LPS诱发ALI模型,乌司他丁组按50000u/kg体重给予乌司他丁静脉注射。各组给药后2h、6h、12h观察丙二醛(MDA)的含量以及HO-1的活性;RT-PCR和Western blot检测HO-1 mRNA和蛋白的表达情况。结果用LPS成功复制出ALI模型,同时,肺组织中MDA含量、HO-1蛋白活性、HO-1 mRNA以及蛋白水平均高于阴性对照组(P均<0.05);乌司他丁+LPS组、乌司他丁+LPS+Hm组肺MDA含量低于LPS模型组,但HO-1活性、mRNA和蛋白水平高于相应LPS组(P均<0.05)。结论乌司他丁对ALI的保护作用可能是通过增强HO-1活性、上调mRNA和蛋白表达水平而实现的。更多还原

【Abstract】 Objective To study the effect of Ulinastatin on hemeoxygenase(HO)-1 expression in acute lung jury(ALI) rats,and thus.Methods Experimental rats were randomly divided into five groups:control group,LPS group,Ulinastatin+LPS group,LPS+Ulinastatin+Hemin gourp and LPS+Ulinastatin+zinc protoporphyrin gourp.The structure of the lung,MDA content,HO-1 activity,mRNA of HO-1 and protein expression were detected.Results The ALI model was successfully constructed by LPS,and the MDA content,HO-1 activity,mRNA and proteins were higher than those in control group(P<0.05).The degree of lung injury and the MDA content were lower,and the activity and the expression of HO-1 in Ulinastatin+LPS and Ulinastatin+LPS+Hemin group were higher than those in LPS group(P<0.05).The degree of lung injury and MDA content were higher,the activity and the expression of HO-1 were lower in LPS+Ulinastatin+ZnPP than those in LPS group(P<0.05).Conclusion Ulinastatin attenuates LPS-induced ALI may be mediated through upregulation Of HO-1 activity,mRNA and protein expression.更多还原