【摘要】 目的:观察孕期和哺乳期D-半乳糖暴露对仔鼠脂质过氧化、听力功能和耳蜗毛细胞形态结构的影响,阐明其与仔鼠听力障碍-机体脂质过氧化增强之间的关系。方法:12只成功受孕大鼠随机分为D-半乳糖组和对照组,每组6只。自妊娠6d起,对照组孕鼠给予普通饲料,D-半乳糖组孕鼠给予普通饲料配30%D-半乳糖的混合饲料。仔鼠出生后21d,检测仔鼠血清D-半乳糖、全血超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)和血浆丙二醛(MDA)含量变化,检测畸变产物耳声发射(DPOAE)情况,应用硝酸银染色全耳蜗铺片观察仔鼠毛细胞形态学变化。结果:孕期和哺乳期D-半乳糖暴露致仔鼠全血SOD、GSH-Px和GSH水平明显低于对照组(P<0.05),D-半乳糖组仔鼠血浆MDA水平高于对照组(P<0.05);D-半乳糖组仔鼠在各频率上DPOAE幅值均低于对照组(P<0.05);D-半乳糖组仔鼠耳蜗外毛细胞结构受损,且数目缺失。结论:D-半乳糖可诱导仔鼠氧化损伤和听觉发育损伤。更多还原

【Abstract】 Objective To observe the influence of pregnant and lactational D-galactose exposure in lipid peroxidation,hearing function and morphology and structures of cochlear hair cells in newborn rats,and to clarify the relation with cochlear hair cell injury,hearing loss and increased lipid peroxidation.Methods 12 pregnant rats were randomly devided into control group(n=6) and D-galactose group(n= 6) from gestational day 6,the rats in control group were fed on standard rodent diet,and the rats in D-galactose group were fed on standard rodent diet supplemented with 30% D-galactose.On postnatal day 21,the blood levels of galactose,superoxide dismutase(SOD),glutathion(GSH),glutathione peroxidase(GSH-Px) and malondialdethyde(MDA) in newborn rats were measured.Then the distortion product otoacoustic emission(DPOAE) was examined.The temporal bones were then removed and the morphological changes were detected by silver nitrate staining.Results The levels of blood SOD,GSH-Px and GSH were lower in newborn rats after using D-galactose during pregnancy and lactation compared with control group(P<0.05),and the plasma MDA level was higher than that in control group(P<0.05).In addition,the DPOAE threshold shifts at different frequencies were lower after using D-galactose during pregnancy and lactation compared with control group(P<0.05).The structures of cochlea hair cells were impaired and the number of hair cells was decreased in D-galactose group.Conclusion D-galactose exposure may cause oxidative and cochlear function damage in newborn rats.更多还原