【摘要】 目的分析中国汉族人群HPA-1～6、15系统的基因多态性,研究中国南、北方汉族人群HPA-1～6、15的基因分布。方法采用Luminex结合序列特异性寡核苷酸探针(flow-sequence specific oligonucleotide probes,FLOW-SSO)方法对2 458名深圳巿机采血小板无偿捐献者(其中南方人群1 554人,北方人群904人)进行HPA-1～6,15系统基因分型。采用聚合酶链反应-序列特异性引物(PCR-sequence specific primer,PCR-SSP)方法对有疑问的标本及罕见抗原(如HPA-1b/1b、2b/2b、5b/5b等)做进一步确认。结果中国汉族人群中HPA-1～6、15基因频率分别为,HPA-1a 0.991 7,HPA-1b 0.008 3,HPA-2a 0.955 2,HPA-2b 0.044 8,HPA-3a 0.558 6,HPA-3b 0.441 4,HPA-4a0.998 0,HPA-4b 0.002 0,HPA-5a 0.986 2,HPA-5b 0.013 8,HPA-6a 0.985 6,HPA-6b 0.014 4,HPA-15a 0.545 2,HPA-15b 0.454 8。南、北方汉族人群HPA-1～6、15比较,在HPA-1和HPA-3系统(χ2=15.032 0、5.418 8,P<0.05);基因频率分布差异有统计学意义。经χ2检验,符合Hardy-Weinbery遗传定律。结论中国汉族人群中HPA-3和HPA-15杂合程度最高,在中国南北方汉族人群中HPA-1和HPA-3系统基因多态性分布存在明显的地域差异。为了给免疫性血小板减少症患者提供相合血小板输注,在中国建立血小板供者资料库是必要的。更多还原

【Abstract】 Objective To analyze human platelet antigens(HPA)1-6,15 polymorphism,and study the allele frequencies in the North Chinese Han and South Chinese Han.Methods The genotypes of HPA1-6,15 systems were performed by FLOW-SSO for 2 458 samples.Results The gene frequencies were 0.991 7,0.008 3,0.955 2,0.044 8,0.558 6,0.441 4,0.998 0,0.002 0,0.986 2,0.013 8,0.985 6,0.014 4,0.545 2,0.454 8 for HPA-1a,HPA-1b,HPA-2a,HPA-2b,HPA-3a,HPA-3b,HPA-4a,HPA-4b,HPA-5a,HPA-5b,HPA-6a,HPA-6b,HPA-15a,HPA-15b,respectively.The phenotype frequencies of HPA1-6,15 in the South Chinese Han was compared to the frequencies in the north Chinese Han.There is significant deviation from Hardy-Weinbery equilibrium within HPA-1,3 in the south Chinese Han and north Chinese Han(χ2=15.032 0、5.418 8,P<0.05).There was a good fit to Hardy-Weinbery equilibrium within each group.Conclusion The highest heterozygotes are HPA-3 and HPA-15,which indicate that the original difference of HPA-1 and HPA-3 in South and North of China.In order to provide HPA-matched platelet transfusion in alloimmune thrombocytopenic patients,the building of China HPA-typed plateletpheresis donor registry is very useful.更多还原